Web22 Channelrhodopsin-assisted circuit mapping (CRACM) for functional is a precision technique 23 mapping of long-range neuronal projections between anatomically and/or genetically identified 24 groups of neurons. Here, we describe how to utilize CRACM to map auditory brainstem 25 WebOct 19, 2024 · To examine whether the LHA-projecting MPL SST neurons are responsible for the observed self-grooming behavior, we injected retrogradely transported AAV expressing Cre-dependent ChR2 (retroAAV-DIO-ChR2-mCherry) into the LHA of SST-Cre mice, resulting in the specific expression of ChR2-mCherry in the LHA-projecting MPL …
Descending Projections from the Basal Forebrain to the Orexin …
WebOct 1, 2024 · Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such networks. Using this combination of methods, originally termed channelrhodopsin … Webas well as GABAergic neurons in the DRN with channelrhodop- sin-2 (ChR2)-assisted circuit mapping (CRACM) (Petreanu et al., 2007). RESULTS Serotonergic Neurons in DRN Receive Monosynaptic Glutamatergic Input from LHb The lateral habenula (LHb) is implicated in emotional regulation, whichispossiblymediatedbytheLHb … thomson n15c8bk2t driver
Channelrhodopsin-2 Assisted Circuit Mapping in the …
WebSep 2, 2024 · We then used channelrhodopsin (ChR2)-assisted circuit mapping (CRACM) to inform a more detailed understanding of the synaptic output pathways by which SCN … WebWe applied this technique, ChR2- assisted circuit mapping ( CRACM), to map long- range callosal projections from layer ( L) 2/3 of the somatosensory cortex. L2/3 axons connect … WebMay 27, 2024 · Experimental setup for channelrhodopdsin-2 assisted circuit mapping (CRACM). (a) ChR2 expression in dorsal horn neurons can be achieved by viral injection either directly into the spinal cord to transduce dorsal horn neurons, or into the brain to transduce projection neurons via retrograde transport. Importantly this can be done using … thomson n15c8bk2t fiche technique